Short-Form Thymic Stromal Lymphopoietin (sfTSLP) Is the Predominant Isoform Expressed by Gynaecologic Cancers and Promotes Tumour Growth

Thymic stromal lymphopoietin (TSLP) is an epithelial cell derived cytokine belonging to the IL-7 family and a key initiator of allergic inflammation. Two main isoforms TSLP, classified as long- (lfTSLP) short-form (sfTSLP), have been reported in human, but their expression patterns role(s) cancers are not yet clear. mRNA was examined by isoform-specific RT-PCR RNA situ hybridisation. Epigenetic regulation investigated chromatin immunoprecipitation-PCR bisulfite sequencing. Tumour progression gene overexpression, viability assay, cancer organoid culture transwell invasion. Signals were proteome profiler protein array RNA-sequencing. With use primers probes, we uncovered that only sfTSLP expressed lines tumour tissues human ovarian endometrial cancers. We also showed epigenetic sfTSLP: transcription regulated histone acetylation at promoters cells, whereas silencing transcripts promoter DNA methylation cells. In vitro study ectopically overexpressing promoted growth Human phosphokinase application demonstrated overexpression activated phosphorylation multiple intracellular kinases (including GSK3α/β, AMPKα1, p53, AKT1/2, ERK1/2 Src) cells context-dependent manner. further impact on transcriptome RNA-sequencing found EFNB2 PBX1 downregulated suggesting role sfTSLP-mediated growth. conclusion, predominantly promotes